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目的研究结核分枝杆菌Ag85A/B嵌合DNA疫苗治疗小鼠敏感结核病的效果。方法用结核分枝杆菌标准株H37Rv尾静脉注射50只雌性BALB/c小鼠后,将小鼠随机均匀地分为5组,感染后第3 d开始,分别用生理盐水(A组)、pVAX1载体(B组)、利福平(C组)、草分枝杆菌F.U.36注射液(D组)、Ag85A/B嵌合DNA疫苗(E组)治疗,每2周肌肉注射1次DNA疫苗,共3次。在治疗结束后2周,杀鼠,取小鼠肺和脾观察病理改变、称取重量、做菌落计数。结果与A组比较,D组、E组肺脏病变有不同程度减轻,病变局限,3/5区域肺泡结构完整,清晰,细胞分布均匀。与A组相比,C、D、E组肺脏菌落数依次减少2.11 log、0.76 log、0.81 log;肝脏菌落数依次减少2.11 log、0.74 log、1.11 logs(P<0.01)。结论 Ag85A/B嵌合DNA对小鼠敏感结核病具有较好的治疗效果。
Objective To study the effect of Mycobacterium tuberculosis Ag85A / B chimeric DNA vaccine in the treatment of sensitive tuberculosis in mice. Methods Fifty female BALB / c mice were injected into the tail vein of the standard strain of Mycobacterium tuberculosis H37Rv. The mice were randomly divided into five groups randomly. The mice were sacrificed at day 3 after infection with normal saline (group A), pVAX1 (Group B), rifampin (group C), Mycobacterium phlei FU36 injection (group D) and Ag85A / B chimeric DNA vaccine (group E). DNA vaccine was injected intramuscularly every two weeks, A total of 3 times. 2 weeks after treatment, kill mice, take mice lung and spleen to observe pathological changes, weighed, do colony count. Results Compared with group A, the lung lesions in group D and group E were relieved to varying degrees, and the lesions were limited. The alveolar structure in 3/5 region was complete and clear, and the cells were evenly distributed. Compared with group A, the number of lung colonies in groups C, D and E decreased 2.11 log, 0.76 log and 0.81 log in turn, and the number of liver colonies decreased 2.11 log, 0.74 log and 1.11 logs, respectively (P <0.01). Conclusion Ag85A / B chimeric DNA has good therapeutic effect on sensitive tuberculosis in mice.