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目的:探讨健脑益智方对脑缺血再灌注损伤后白细胞计数、髓过氧化物酶及细胞间黏附分子-1的影响。方法:采用健康雄性SD大鼠四动脉闭塞法制备大鼠全脑缺血再灌注模型,于全脑缺血20 min,再灌注24 h后,测定外周血白细胞总数、非淋巴细胞数及百分比、淋巴细胞数及百分比;大脑皮质和海马中MPO的活性及海马结构内ICAM-1表达情况。结果:模型组大鼠外周静脉血中的白细胞总数、非淋巴细胞计数及其百分比明显升高(P<0.01),淋巴细胞百分比降低(P<0.01);大鼠脑组织皮质区和海马区的MPO含量均明显升高(P<0.01,P<0.05);ICAM-1阳性神经元平均光密度值高于假手术组(P<0.01),平均灰度值低于假手术组(P<0.01)。与模型组比较,阿司匹林干预后造模组、中药干预后造模组及中药治疗组的白细胞总数、非淋巴细胞总数(P<0.01)及其百分比均降低(P<0.05),淋巴细胞百分比升高(P<0.05);MPO的含量均不同程度的降低(P<0.01,P<0.05);ICAM-1阳性神经元平均光密度值均降低(P<0.01,P<0.05),平均灰度值均升高(P<0.01,P<0.05)。结论:健脑益智方可抑制大鼠脑缺血再灌注后缺血脑组织中的白细胞(主要是中性粒细胞)的浸润,下调脑组织中细胞间黏附分子-1的表达,并可能通过以上途径阻断其后的炎症级联反应来达到减轻脑缺血再灌注损伤,促进神经功能的修复。
Objective: To investigate the effect of jiannao yizhi decoction on white blood cell count, myeloperoxidase and intercellular adhesion molecule-1 after cerebral ischemia-reperfusion injury. Methods: The rat model of global cerebral ischemia-reperfusion was established by four-artery occlusion of healthy male Sprague-Dawley rats. The total number of leukocytes, the number of non-lymphocytes and the percentage of non-lymphocytes in the whole brain were measured after 20 minutes of global cerebral ischemia and 24 hours of reperfusion. Lymphocyte number and percentage; MPO activity in the cerebral cortex and hippocampus and ICAM-1 expression in the hippocampal formation. Results: The number of white blood cells, non-lymphocyte count and percentage of peripheral blood in model group were significantly increased (P <0.01), and the percentage of lymphocytes was decreased (P <0.01). The cortical and hippocampal regions (P <0.01, P <0.05). The average optical density of ICAM-1 positive neurons was higher than that of sham operation group (P <0.01), and the average gray value of ICAM-1 positive neuron was lower than that of sham operation group ). Compared with the model group, the total number of leukocytes, the total number of non-lymphocytes (P <0.01) and the percentage of non-lymphocytes (P <0.05) in model group and traditional Chinese medicine group after intervention with aspirin were lower (P <0.01, P <0.05). The average optical density of ICAM-1 positive neurons decreased (P <0.01, P <0.05), the mean gray level Value increased (P <0.01, P <0.05). Conclusion: Jiannao Yizhi can inhibit the infiltration of leukocytes (mainly neutrophils) in the ischemic brain tissue after cerebral ischemia-reperfusion in rats and down-regulate the expression of intercellular adhesion molecule-1 in brain tissue and may Through the above pathways to block the subsequent inflammatory cascade to reduce cerebral ischemia-reperfusion injury and promote neurological repair.