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以黄瓜(Cucumis sativus L.)成熟瓜红色果皮自交系‘NCG127’(P1)和成熟瓜黄色果皮自交系‘9930’(P2)为试验材料构建F2遗传群体,对成熟瓜红色果皮R基因进行遗传分析和基因定位研究。结果表明,黄瓜成熟瓜红色果皮性状由显性单基因控制,红色对黄色为显性。以256株F2分离群体为试材,应用群体分离分析(BSA)法筛选得到与R基因连锁的20个多态性SSR标记,构建了R基因的分子标记连锁图谱,将R基因定位到黄瓜4号染色体上,物理距离为213.4 kb的区段内,两侧翼标记为UW019319和UW019203,与R遗传距离分别为0.8 cM和0.7 cM。生物信息学分析表明,该区段存在30个预测候选基因。
The F2 genetic population was constructed from the inbred line “Cucumber (Cucumis sativus L.)” inbred line “NCG127” (P1) and the mature melon yellow inbred line ’9930’ (P2). The R gene Conduct genetic analysis and gene mapping studies. The results showed that the cucumber ripe melon red peel trait controlled by a dominant single gene, red to yellow dominant. A total of 256 F2 isolates were used as experimental materials. Twenty SSR markers linked to R gene were screened by population segregation analysis (BSA), and a molecular linkage map of R gene was constructed. The R gene was mapped to Cucumber 4 On the chromosome, the physical distance was 213.4 kb. The wings on both sides were labeled as UW019319 and UW019203, and the genetic distance to R was 0.8 cM and 0.7 cM, respectively. Bioinformatics analysis showed that there are 30 predicted candidate genes in this section.