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目的探讨肿瘤坏死因子相关的凋亡诱导配体(TRAIL)及其死亡受体5(DR5)在巨细胞病毒(MCMV)肝炎新生小鼠肝组织中的表达变化及意义。方法 48只新生小鼠随机分为对照组(24只)与病毒组(24只),病毒组腹腔接种MCMV建立MCMV肝炎模型(MCMV组),对照组腹腔注射等量的无菌生理盐水。聚合酶链反应技术(PCR)检测小鼠肝脏MCMV DNA,ELISA法检测血清丙氨酸转氨酶(ALT),反转录(RT)-PCR法和免疫组织化学法检测肝脏的TRAIL和DR5蛋白及mRNA的表达,并分析它们与ALT的相关性。结果 MCMV组和正常对照组小鼠肝脏组织中均存在TRAIL、DR5蛋白和mRNA的表达;与正常对照组相比,MCMV组TRAIL及DR5mRNA的表达呈增高趋势,第7天达高峰,14 d有所下降。免疫组织化学法显示肝脏TRAIL、DR5蛋白的表达也在病毒感染后第7天达到高峰,且二者与ALT表达水平呈正相关。结论 MCMV肝炎新生小鼠TRAIL及受体DR5在肝脏组织中呈现高表达,且与ALT呈正相关,提示TRAIL及受体DR5通过调控肝脏细胞凋亡参与MCMV肝炎的病理过程。
Objective To investigate the expression and significance of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its death receptor 5 (DR5) in liver tissue of newborn mice with cytomegalovirus (MCMV) hepatitis. Methods 48 neonatal mice were randomly divided into control group (24 mice) and virus group (24 mice). The MCMV mice were intraperitoneally inoculated with MCMV virus to establish MCMV hepatitis model (MCMV group). The control group received intraperitoneal injection of sterile normal saline. The liver of MCMV DNA was detected by polymerase chain reaction (PCR), serum alanine aminotransferase (ALT), reverse transcriptase (RT) -PCR and immunohistochemistry were used to detect the liver TRAIL and DR5 protein and mRNA , And analyzed their association with ALT. Results The expressions of TRAIL, DR5 protein and mRNA were found in the liver of MCMV group and normal control group. Compared with the normal control group, the expression of TRAIL and DR5 mRNA increased in the MCMV group and peaked on the 7th day The decline. Immunohistochemistry showed that the expression of TRAIL and DR5 in liver also peaked on the 7th day after virus infection, and the expression of TRAIL and DR5 was positively correlated with the expression of ALT. Conclusion The expression of TRAIL and DR5 in MCMV hepatitis neonatal mice is highly expressed in liver tissue and positively correlates with ALT, suggesting that TRAIL and DR5 are involved in the pathological process of MCMV hepatitis through regulating hepatic cell apoptosis.