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目的:考察紫菀丸对血热出血模型大鼠血液流变性、凝血时间及肺部的影响。方法:SD大鼠随机分为6组,分别为空白对照组,模型对照组,云南白药组(0.5 g.kg-1),紫菀丸高、中、低(5,2.5,1.25 g.kg-1)剂量组。将供试品按每日20 mL.kg-1的剂量灌胃(ig),2次/d,给药7 d。除空白对照组外,其余组复制血热出血大鼠模型,并比较紫菀丸对其血液流变性、凝血酶时间(TT)、活化部分凝血酶原时间(APTT)、凝血酶原时间(PT)、纤维蛋白含量(FIB)以及肺部的影响。结果:模型对照组较空白对照组有显著性差异,明显升高全血黏度,血浆黏度,TT,APTT明显延长,PT显著缩短,FIB含量显著增加。紫菀丸中剂量组能够显著降低血热出血模型大鼠的全血黏度,血浆黏度,APTT,TT,明显增加FIB的含量。紫菀丸高剂量组能明显缩短TT,紫菀丸低剂量组能显著延长PT。紫菀丸中剂量组依次为全血黏度200 s-1(5.87±0.24)mPa.s,30 s-1(7.60±0.36)mPa.s,5 s-1(13.70±0.51)mPa.s,1 s-1(32.38±2.06)mPa.s,APTT(17.11±0.78)s,TT(30.07±2.00)s,FIB(5.56±0.14)g.L-1;紫菀丸高剂量组为TT(30.74±2.96)s;紫菀丸低剂量组为PT(14.63±1.14)s;与模型对照组比较P<0.05或P<0.01。在肺体征方面,紫菀丸能够改善血热出血大鼠肺部损伤的相关症状,中剂量效果最好,其次是高、低剂量。结论:紫菀丸中剂量组能够改善血热出血大鼠异常的血液流变学指标,通过影响内、外源性凝血酶及纤维蛋白原来达到止、凝血效果,紫菀丸高剂量组对部分血液流变学和凝血指标有一定的效果。紫菀丸剂量组对模型组大鼠肺部的损伤都有一定的治疗作用。
Objective: To investigate the effect of Ziyan Pills on the hemorheology, clotting time and lung in hemorrhagic fever model rats. Methods: The SD rats were randomly divided into 6 groups: blank control group, model control group, Yunnan Baiyao group (0.5 g.kg-1), Ziqi Wan high, middle and low (5,2.5,1.25 g.kg- 1) dose group. The test will be daily doses of 20 mL.kg-1 gavage (ig), 2 times / d, administered 7 d. In addition to the blank control group, the other groups were copied blood hemorrhagic fever rat model, and compared the purple rheumatism, hemorrheology, thrombin time (TT), activated partial prothrombin time (APTT), prothrombin time (PT) , Fibrin content (FIB) and lung effects. Results: Compared with the blank control group, the model control group significantly increased the viscosity of whole blood, the viscosity of plasma, the prolongation of TT and APTT, the shortening of PT and the increase of FIB content. Asterigo pill medium dose group can significantly reduce the whole blood viscosity, plasma viscosity, APTT, TT, increase the content of FIB significantly. Aspirin high-dose group can significantly shorten the TT, purple pills low-dose group can significantly extend the PT. The mean dose of the purple pills was 200 s-1 (5.87 ± 0.24) mPa.s, 30 s-1 (7.60 ± 0.36) mPa.s and 5 s-1 (13.70 ± 0.51) mPa.s, (30.74 ± 2.96) s, TT-1 (30.07 ± 2.00) s, FIB (5.56 ± 0.14) gL-1, s; purple pills low dose group PT (14.63 ± 1.14) s; compared with the model control group P <0.05 or P <0.01. In the aspect of lung sign, Zi Mi Wan can improve the related symptoms of lung injury in blood-heathemorrhagic rats, the middle dose is the best, followed by high and low dose. Conclusion: Ziyan Pills medium dose group can improve abnormal hemorheology in hemorrhagic rats with hemorrhagic fever, reach the clotting effect by affecting the exogenous thrombin and fibrinogen, Change and coagulation index have a certain effect. Ziyan pills dose group on the model group rats lung injury have a certain therapeutic effect.