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目的 探讨核酸体外扩增(polymerase chain reaction,PCR)技术在骨结核病诊断与鉴别诊断中的应用价值。方法 对60例骨结核病标本与30例非骨结核病标本分别进行核酸体外扩增技术,抗酸染色镜检及结核杆菌分离培养法检测结核杆菌,分析影响核酸体外扩增技术结果的相关因素及处理方法。结果 骨结核标本结核杆菌检出阳性率:PCR法83％,抗酸染色镜检法3％,培养法7％。非骨结核标本结核杆菌检出阳性率:PCR法10％,其他方法均未检出,经统计学处理P<0.005,PCR法与镜检法及培养法相比较差异具有显著性。结论 PCR技术是一种快速、敏感、简便、特异的检测骨结核标本结核杆菌的方法,明显优于镜检法及培养法,在骨结核的诊断与鉴别诊断中有重要价值。
Objective To investigate the value of nucleic acid polymerase chain reaction (PCR) in the diagnosis and differential diagnosis of tuberculosis. Methods Sixty specimens of bone tuberculosis and 30 specimens of non-bone tuberculosis were amplified by nucleic acid in vitro. Anti-acid staining microscopy and Mycobacterium tuberculosis isolates were used to detect Mycobacterium tuberculosis. The related factors influencing the results of nucleic acid amplification in vitro were analyzed. method. Results The positive rates of Mycobacterium tuberculosis were 83% in PCR, 3% in acid-fast staining and 7% in culture. The positive rate of non-mycobacterium tuberculosis Mycobacterium tuberculosis: PCR 10%, no other methods were detected, the statistical treatment of P <0.005, PCR and microscopy and culture were significantly different. Conclusion PCR is a rapid, sensitive, simple and specific method for the detection of tuberculosis Mycobacterium tuberculosis, which is obviously superior to microscopy and culture methods. It is of great value in the diagnosis and differential diagnosis of tuberculosis.