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本研究探讨Notch配体Delta-like 1(Dll1)对小鼠骨髓细胞来源的树突状细胞(dendritic cell,DC)分化及其抗原呈递功能的影响。在GM-CSF和IL-4存在条件下,用OP9-Dll1和OP9-GFP细胞分别与小鼠骨髓细胞共培养8天,经肿瘤抗原刺激成熟。用流式细胞仪检测DC表面MHCII、CD80和CD86的表达情况,ELISA法检测肿瘤抗原刺激后DC培养上清细胞因子IL-12和IL-10的水平,通过混合T淋巴细胞反应观察DC对T细胞的促增殖能力。结果表明:与GFP组相比,Dll1组的小鼠骨髓来源的树突状细胞明显增多(p<0.05)。肿瘤抗原刺激后,Dll1组DC表面MHCII、CD80和CD86表达量更高。DC分泌的IL-12水平显著高于对照组(p<0.05),而IL-10的水平显著低于对照组(p<0.01)。Dll1组DC具有更强的促T细胞增殖活性。结论:OP9-Dll1促进小鼠骨髓细胞向DC分化并增强其抗原呈递功能。
This study was designed to investigate the effects of Notch ligand Delta-like 1 (Dll1) on dendritic cell (DC) differentiation and antigen presenting function of mouse bone marrow cells. In the presence of GM-CSF and IL-4, OP9-D111 and OP9-GFP cells were co-cultured with mouse bone marrow cells for 8 days and stimulated with tumor antigens to mature. The expression of MHCII, CD80 and CD86 on DCs were detected by flow cytometry. The levels of IL-12 and IL-10 in supernatants of DCs stimulated by tumor antigen were detected by ELISA. The effect of DC on T Proliferation of cells. The results showed that compared with GFP group, the number of bone marrow-derived dendritic cells in Dll1 group increased significantly (p <0.05). Tumor antigen stimulation, Dll1 DC surface expression of MHCII, CD80 and CD86 higher. DC secreted IL-12 levels were significantly higher than the control group (p <0.05), while the level of IL-10 was significantly lower than the control group (p <0.01). Dll1 group DC has a stronger pro-T cell proliferation activity. Conclusion: OP9-D111 promotes the differentiation of mouse bone marrow cells to DC and enhances its antigen presenting function.