论文部分内容阅读
为了解低氧对肺内血管内皮生长因子（ＶＥＧＦ）基因表达的影响及ＶＥＧＦ在肺动脉高压发生中的作用，以常压低氧建立大鼠肺动脉高压模型，采用Ｅｌｉｓａ法检测大鼠肺动脉血血清ＶＥＧＦ的含量改变，以体外转录并用ＤＩＧ－ＵＴＰ标记的ＶＥＧＦｃＲＮＡ探针进行肺组织原位杂交，检测大鼠肺内ＶＥＧＦｍＲＮＡ表达的变化。结果发现：低氧３周后，大鼠形成明显的肺动脉高压；大鼠肺动脉血血清中ＶＥＧＦ含量在低氧３周组为４２０．３±７３．１ｐｇ／ｍｌ，明显高于对照组的３２２．２±５８．１ｐｇ／ｍｌ（Ｐ＜０．０１）；原位杂交显示，低氧大鼠肺小动脉管壁出现明显的ＶＥＧＦｍＲＮＡ表达，说明低氧在引起肺动脉高压的同时可刺激肺小动脉管壁，使ＶＥＧＦｍＲＮＡ表达增强，导致ＶＥＧＦ的合成和分泌增加，后者可能在低氧性肺动脉高压发生过程中起一定的作用。
To investigate the effect of hypoxia on the gene expression of vascular endothelial growth factor (VEGF) and the role of VEGF in the pathogenesis of pulmonary hypertension in rats, pulmonary hypertension model was established by hypobaric hypoxia and Elisa method was used to detect the expression of VEGF The changes of VEGF mRNA expression in the lungs of rats were detected by in situ hybridization and in vitro transcription of VEGFcRNA probe labeled with DIG-UTP. The results showed that: After hypoxia for 3 weeks, the rats developed obvious pulmonary hypertension; the content of VEGF in the pulmonary arterial blood serum of rats was 420.3 ± 73.1 pg / ml in the 3rd week of hypoxia, which was significantly higher than that of the control group. 2 ± 58.1pg / ml (P <0.01). In situ hybridization showed that the expression of VEGFmRNA in the hypoxia rat pulmonary arterioles showed that hypoxia could stimulate pulmonary arterioles Wall, so that VEGF mRNA expression increased, resulting in increased VEGF synthesis and secretion, which may play a role in hypoxic pulmonary hypertension.