用甜叶菊“中山一号”嫩茎或叶柄为外植体 ,MS为基本培养基 ,在附加BA(0 5mg/L) +NAA(0 2mg/L)的分化培养基上 ,分化率为85 % ;继代增殖在附加ZT(1 5mg/L) +NAA(0 1mg/L)的培养基上 ,每3～4周可增殖5～6倍 ;在附加BA(0 1mg/L) +NAA(0 5mg/L) +IBA(0 5mg/L)的生根培养基上 ,生根效果最好 ,出瓶前须炼苗2～3d ,生根苗移栽成活率在90 %以上 Using the stems or petiole of “Zhongshan No.1” of Stevia rebaudiana as explants, MS was taken as the basic medium. The differentiation rate was 85 on the differentiation medium supplemented with BA (0 5 mg / L) and NAA (0 2 mg / L) %. The proliferation of subculture medium was increased 5 ~ 6 times every 3 ~ 4 weeks on medium supplemented with ZT (15mg / L) and NAA (0 1mg / L) (0 5mg / L) + IBA (0 5mg / L) rooting medium, the best rooting effect, the flask should be removed before the bottle 2 ~ 3d, rooting seedlings transplanted survival rate of more than 90%