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目的以黄独脱毒苗为试材,研究不同因素对黄独带芽茎段芽增殖和生根的影响,以期对黄独脱毒苗的快繁技术进行优化。方法采用植物组织培养的方法进行茎尖培养和快繁研究,采用RT-PCR法对茎尖脱毒植株进行病毒检测。结果黄独脱毒苗带芽茎段的最佳培养基是MS+KT 2 mg/L+6-BA 1 mg/L+NAA 0.5 mg/L;黄独脱毒苗带芽茎段增殖的最佳蔗糖质量浓度和琼脂质量浓度分别是30和0 g/L;黄独脱毒苗带芽茎段生根的最佳培养基是1/2 MS+IBA 0.1 mg/L+NAA 0.5 mg/L+PP_(333)1 mg/L;黄独试管苗移栽的最好基质是珍珠岩:蛭石(2:1);黄独试管苗移栽时最佳的PP_(333)质量浓度是50 mg/L。结论首次成功建立了黄独脱毒苗的快繁技术,为黄独脱毒苗的工厂化生产奠定了技术基础。
Objective To study the effects of different factors on the proliferation and rooting of buds of buds of Buds of Pseudomonas aeruginosa in order to optimize the technique of rapid propagation of virus - free seedlings of Pseudostellaria. Methods Tissue culture method was used to cultivate and propagate shoot tips, and the virus was detected by RT-PCR. Results The best culture medium for the stems and leaves of the virus-free seedlings was MS + KT 2 mg / L + 6-BA 1 mg / L + NAA 0.5 mg / L. The optimal medium for sucrose concentration and agar concentration were 30 and 0 g / L, respectively. The optimum culture medium for the rooting of the seedlings with buds was 1/2 MS + IBA 0.1 mg / L + NAA 0.5 mg / L + PP 333 333 mg / L. The best substrate for the transplanting of yellow detached tube seedlings was perlite and vermiculite (2: 1). The optimum concentration of PP 333 in the yellow detached tube seedlings was 50 mg / L. Conclusion For the first time, the rapid propagation of virus-free vaccine was established successfully, which laid the technical foundation for the industrialized production of yellow virus-free vaccine.