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以国家品种资源库编目入库的云南地方稻种资源 61 2 1份为材料 ,以 31个分类、形态及产量性状为基本数据研究了云南地方稻种资源的核心种质取样方案。取样方案包括分组原则、组内取样比例的确定和组内取样方法 ,分组原则为按丁颖分类体系、程王分类体系、云南稻作生态区、行政地区和单一性状分组及不分组的大随机 ;组内取样比例的确定有平方根法、对数法、遗传多样性法和简单比例法 ;组内取样采用随机法和聚类法。结果表明 ,遗传多样性指数、表型方差、表型频率方差、变异系数和表型保留比例等 5个参数可作为检验各取样方案所得的核心种质的指标 ,通过检验可知 ,以丁颖和程王两种分类体系为分组原则、以平方根或对数法确定组内取样比例采用聚类法在组内进行取样是比较好的取样方案
A total of 61 21 rice landraces from Yunnan were used as materials to study the sampling plan of core germplasm resources of Yunnan local landraces based on 31 taxonomic, morphological and yield characters. The sampling plan includes the principle of grouping, the determination of sampling proportion in group and sampling method in group. The grouping principle is classified according to Dingying classification system, Wangcheng classification system, Yunnan rice planting ecological zone, administrative region and single trait grouping and non-grouping large random The sampling ratio in the group was determined by square root method, logarithmic method, genetic diversity method and simple proportional method. The random sampling method and cluster method were used for sampling in the group. The results showed that five parameters of genetic diversity index, phenotypic variance, phenotypic frequency variance, coefficient of variation and phenotypic retention ratio could be used as indexes to test the core germplasm obtained from each sampling scheme. According to the test, Wang Cheng two classification system for the principle of grouping, square root or logarithmic method to determine the proportion of samples within the cluster cluster sampling method is a good sampling plan