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目的观察多壁碳纳米管(multi-walled nanotubes,MWCNTs)对DNA超螺旋构型的影响和氧化损伤。方法选择不同浓度多壁碳纳米管(50～500μg/ml)与pBR322质粒DNA及小牛胸腺DNA(ct-DNA)作用,采用质粒DNA凝胶电泳和圆二色谱分析。结果多壁碳纳米管能够引起pBR322质粒DNA的损伤,其DNA损伤率(16%～42%)呈剂量-反应关系,多壁碳纳米管高浓度(500μg/ml)时DNA降解成线性条带。在活性氧清除剂-维生素C的作用下,DNA损伤率可由45.24%降低到18.64%。多壁碳纳米管可削弱DNA碱基之间的堆积作用,导致DNA解链。结论多壁碳纳米管可通过活性氧介导DNA损伤。
Objective To investigate the effect of multi-walled nanotubes (MWCNTs) on DNA supercoiled configuration and oxidative damage. Methods Different concentrations of multi-walled carbon nanotubes (50-500μg / ml) were used in combination with pBR322 plasmid DNA and calf thymus DNA (ct-DNA). DNA gel electrophoresis and circular dichroism analysis were used. Results Multi-walled carbon nanotubes (MWCNTs) could induce the DNA damage of pBR322. The rate of DNA damage (16% -42%) was dose-response. The DNA was degraded into linear bands . Under the action of active oxygen scavenger-vitamin C, the DNA damage rate decreased from 45.24% to 18.64%. Multi-walled carbon nanotubes can weaken the accumulation of DNA bases, leading to DNA melting. Conclusion Multi-walled carbon nanotubes can mediate DNA damage through reactive oxygen species.