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目的探究大气细颗粒物(PM_(2.5))对肥大细胞的生物学效应。方法采用智能中流量空气总悬浮微粒采样器采集重庆市内非工业区空气中PM_(2.5),以不同浓度(0、12.5、25.0、50.0、100.0、200.0、400.0、800.0μg/m L)PM_(2.5)混悬液作用于人肥大细胞系LAD2,采用微板法检测LAD2细胞β-氨基己糖苷酶的释放水平;采用ELISA法检测LAD2细胞释放组胺、白细胞介素4(interleukiin-4,IL-4)以及活性氧(reactive oxygen species,ROS)水平。结果终浓度为25.0μg/m L的PM_(2.5)刺激后,LAD2细胞分泌并释放入细胞上清的β-氨基己糖苷酶、组胺及IL-4的水平高于0μg/m L PM_(2.5)组[(23.78±3.98)%vs(14.05±1.12)%,(12.32±0.18)vs(9.72±0.23)μg/m L,(267.63±7.97)vs(154.25±7.71)pg/m L,P<0.01];12.5~100.0μg/m L PM_(2.5)刺激后,LAD2细胞产生ROS水平逐渐升高,终浓度为25.0μg/m L的PM_(2.5)刺激LAD2细胞后产生的ROS水平高于0μg/m L PM_(2.5)组[(7.99±0.29)vs(5.88±0.49)ng/m L,P<0.01]。结论 PM_(2.5)可能通过氧化应激致使肥大细胞产生ROS,引起肥大细胞产生细胞因子和炎症因子。
Objective To investigate the biological effects of fine particulate matter (PM_ (2.5)) on mast cells. Methods PM_ (2.5) in the air of Chongqing non-industrial zone was collected by using intelligent mid-flow air total suspended particle sampler. The PM_ (2.5), PM_ (2.5) suspension on human mast cell line LAD2. The release of β-hexosaminidase was detected by microplate method. The levels of histamine and interleukin-4 (IL-4) IL-4) and reactive oxygen species (ROS) levels. Results After stimulation with PM 2.5 at a final concentration of 25.0 μg / mL, the level of histamine and IL-4 secreted by LAD2 cells and released into the cell supernatant was higher than that of 0 μg / mL PM_ ( (23.78 ± 3.98)% vs (14.05 ± 1.12)%, (12.32 ± 0.18) vs (9.72 ± 0.23) μg / m L, (267.63 ± 7.97) vs (154.25 ± 7.71) pg / P <0.01]. The level of ROS in LAD2 cells was increased after 12.5 ~ 100.0μg / m L PM 2.5 stimulation, and the level of ROS produced by LMP2 cells stimulated by PM 2.5 at a final concentration of 25.0μg / mL (7.99 ± 0.29 vs 5.88 ± 0.49 ng / m L, P <0.01] in 0 μg / m L PM 2.5 group. Conclusion PM_ (2.5) may cause the mast cells to produce ROS through oxidative stress and cause the mast cells to produce cytokines and inflammatory cytokines.