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目的 探讨面神经再生微环境及神经营养素 3(NT 3)对神经细胞的作用。方法 将新西兰兔一侧面神经干横切断后置入硅胶再生室 ,再将13 1I NT 3或12 5I NT 3或13 1I 人血清白蛋白 (HSA)(10 μL/只 ,约 7 4MBq)注入再生室 ,取注入13 1I NT 3或13 1I HSA的兔分别在注药后不同时刻行头部冠状位平面显像 ,取注入12 5I NT 3兔的面神经干、脑干和对侧面神经干测定其转运率。另取置入再生室的兔同时在再生室注入 1mgNT 3或 5 4mgHSA和 7 4MBq13 1I NT 3后 ,不同时刻行头部冠状位平面显像。结果 注药后 4h有 4 0 5 %的12 5I NT 3转运到面神经干中 ,12h为最高峰 (2 7 0 4% ) ;8h有9 85 %转运至脑干 ,2 4h转运至脑干的量达 41 18% ,但13 1I HSA在面神经中无转运 ,且13 1I NT 3在面神经的转运受NT 3的明显抑制。结论 NT 3在面神经中存在受体介导逆行转运。
Objective To investigate the effects of facial nerve regeneration microenvironment and neurotrophin 3 (NT 3) on neural cells. Methods New Zealand rabbits were transected one side of the facial nerve trunk and then placed in a silica gel regeneration chamber. Then 13 1I NT 3 or 125I NT 3 or 131I human serum albumin (HSA) (10 μL / only, about 74MBq) The rabbits were injected with 131I NT3 or 131IHSA at different time points after injection, respectively. Coronal plane imaging was performed on the facial nerve stem, brainstem and contralateral nerve trunk injected into 125I NT3 rabbits Transit rate. In addition, the rabbits placed in the regeneration chamber were simultaneously implanted with 1 mgNT 3 or 54 mg HSA and 74MBq13 1I NT 3 in the regeneration chamber, and the coronal plane imaging was performed at different time points. RESULTS: 4h 5% of 125I NT 3 translocated into the facial nerve trunk 4h after injection, with the highest peak at 12h (2 704%); 9 85% of the 12h transport to the brainstem at 8h, and transport to the brain stem at 24 h However, 131 I HSA did not transport in the facial nerve and the transport of 13 1 NT 3 in the facial nerve was significantly inhibited by NT 3. Conclusion There is receptor-mediated retrograde transport of NT 3 in the facial nerve.