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目的探讨碱性磷酸酶组化染色在克罗恩病(CD)诊断中的价值。方法应用BCIP/NBT组化染色检测手术切除的15例克罗恩病和10例溃疡性结肠炎中碱性磷酸酶活性表达水平。结果15例克罗恩病中未受损的肠黏膜表面刷状缘呈紫蓝色BCIP/NBT为(+),而10例溃疡性结肠炎BCIP/NBT均(-)。结论BCIP/NBT组化染色对诊断克罗恩病具有一定的价值。肠黏膜活检中HE常规染色结合BCIP/NBT组化染色可提高CD的诊断正确率。
Objective To investigate the value of alkaline phosphatase staining in the diagnosis of Crohn’s disease (CD). Methods BCIP / NBT histochemical staining was used to detect the expression of alkaline phosphatase activity in 15 cases of Crohn’s disease and 10 cases of ulcerative colitis. Results The intact brush border of intestinal mucosa in 15 cases of Crohn ’s disease was purple blue BCIP / NBT (+), while 10 cases of ulcerative colitis BCIP / NBT were (-). Conclusion BCIP / NBT histochemical staining for the diagnosis of Crohn’s disease has a certain value. Intestinal mucosal biopsy HE routine staining combined with BCIP / NBT histochemical staining can improve the diagnostic accuracy of CD.