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对农垦５８Ｓ（Ｏｒｙｚａｓａｔｉｖａｓｐ．ｊａｐｏｎｉｃａ）／大黑矮生标记基因系ＦＬ２组合组建可育集团和不育集团，并以亲本为对照进行了ＲＦＬＰ、ＲＡＰＤ和双引物ＲＡＰＤ分析，结果第１２染色体上的一个单拷贝标记Ｇ２１４０与光敏核不育基因连锁遗传，二者间的遗传图距为１４．１ｃＭ（ｃｅｎｔｉｍｏｒｇａｎ）。在筛选过的１０４０个随机单引物和１９０个双引物中，仅引物ＯＰＡＵ１０扩增出与光敏核不育基因连锁的１．５ｋｂＤＮＡ片段，回收、克隆该ＤＮＡ片段并制备探针，将其转换成共显性的ＲＦＬＰ标记并命名为ＯＰＡＵ１０１５００。分离群体连锁分析表明该标记与标记Ｇ２１４０紧密连锁，将农垦５８Ｓ的一对光敏核不育基因定位于第１２染色体上。
The fertile and sterile groups were established by the combination of Oryzasativasp.japonica and FL2, and RFLP, RAPD and two-primer RAPD analysis were carried out with their parents as controls. As a result, one The single-copy marker G2140 was linked to the photo-sensitive genic male sterile gene with a genetic distance of 14.1 cM (centimorgan). Of the 1040 random primers and 190 double primers screened, only the primer OPAU10 amplified a 1.5 kb DNA fragment linked to the photogene-sensitive genic male sterility gene. The DNA fragment was recovered and cloned, and the probe was prepared and converted into Co-dominant RFLP marker and named OPAU101500. A linkage analysis of segregating populations showed that the marker was closely linked with the marker G2140 and a pair of photo-sensitive genic male sterility genes of Nongken 58S were mapped on chromosome 12.