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目的研究中药合剂“银菘香”的抗多种病毒活性及其潜在机制。方法检测该复方在体外对甲型流感病毒FM1株(HPR8株(H1N1亚型)、副流感病毒1型(PIV-1)、单纯疱疹病毒1型(HSV-1)、呼吸道合胞病毒(RSV)、柯萨奇病毒B族4型(Cox B4)的抗病毒作用,并通过液相蛋白芯片技术检测“银菘香”对流感病毒诱导的A549细胞的11种炎性因子释放的影响,分析其潜在作用机制。结果研究显示“银菘香”对上述5种病毒均具有一定的抑制作用;“银菘香”可显著降低流感病毒诱导的A549细胞多种细胞因子如IL-2、IL-4、IL-5、IL-6、IL-18、TNF-α、IFN-g和GM-CSF的释放(P<0.05,P<0.01)。结论“银菘香”具抗一定病毒活性,其潜在机制可能与其抑制病毒诱导的多种炎症因子释放,减轻炎症损伤有关。
Objective To study the anti-multi-virus activity and its potential mechanism of traditional Chinese medicine mixture Yin Yin. Methods The compound was tested in vitro against FM1 strain (HPR8 strain (H1N1), parainfluenza virus type 1 (PIV-1), herpes simplex virus type 1 (HSV-1), respiratory syncytial virus ) And Cox B4 were detected by flow cytometry and the effect of “Yin Xiang Xiang” on the release of 11 inflammatory cytokines in A549 cells induced by influenza virus , The potential mechanism of action was analyzed.Results The results show that “Yin Xiang Hong ” on the above five kinds of viruses have a certain degree of inhibition; “Yin Xiang Hong ” can significantly reduce the influenza virus-induced A549 cells a variety of cytokines such as IL-2, IL-4, IL-5, IL-6, IL-18, TNF-α, IFN-γ and GM- "With a certain anti-virus activity, the underlying mechanism may be related to its inhibition of virus-induced release of a variety of inflammatory cytokines, reduce inflammation damage.