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目的 比较不同厂家Muller-Hinton (MH)培养基及不同药敏试验方法用于检测替加环素体外对鲍曼不动杆菌的抗菌活性.方法 分别用Oxoid MH Agar(Oxoid MHA),Oxoid MH Broth+1.5％ Oxoid Agar(Oxoid MHB+ Agar),Becton Dickinson BBL MH ⅡAgar(BD MHA)及bioMérieux MH Ⅱ Agar (bioMérieux MHA)4种培养基,用琼脂稀释法和纸片扩散法,用Oxoid MHB培养基微量稀释法,对临床分离的160株鲍曼不动杆菌进行替加环素体外敏感性进行检测.同时,用原子吸收光谱测试法测定不同培养基钙、铜、铅、镁、镉、锰、铁、镍和锌离子浓度.结果 纸片扩散法/琼脂稀释法采用Oxoid MHA,Oxoid MHB + Agar,BD MHA及bioMérieux MHA 4种培养基所测定鲍曼不动杆菌对替加环素的敏感率分别为34.4％/53.8％,100.0％/96.9％,63.8％/62.5％,35.6％/29.4％.用Oxoid MHB微量稀释法测定替加环素的敏感率为91.3％.结论 纸片扩散法检测鲍曼不动杆菌对替加环素的体外敏感性时,用Oxoid MHB+1.5％ Agar培养基的检测结果较其他MHA培养基的结果与微量肉汤稀释法结果最为接近.“,”Objective To evaluate the Muller-Hinton (MH) mediums from different manufactures and the differences in the activity of tigecycline against Acinetobacter baumannii by different susceptibility test methods in vitro.Methods Testing the activity of tigecycline against 160 strains of clinical isolated Acinetobacter baumannii in vitro by microdilution method when choosing Oxoid MH Broth medium and by agar dilution method or disk diffusion method when choosing Oxoid MH Agar (Oxoid MHA),Oxoid MH Broth + 1.5％ Oxoid Agar(Oxoid MHB + Agar),Becton Dickinson BBL MH Ⅱ Agar(BD MHA) and bioMérieux MH Ⅱ Agar(bioMérieux MHA),respectively.Using atomic absorption spectroscopy method to determine the concentration of calcium,copper,lead,magnesium,cadmium,manganese,iron,nickel and zinc in different medium,simultaneously.Results The susceptibility of tigecycline to Acinetobacter baumannii are 34.4％/53.8％,100.0％/96.9％,63.8％/62.5％,35.6％/29.4％ by agar dilution method or disk diffusion method when using Oxoid MHA,Oxoid MHB + Agar,BD MHA and bioMérieux MHA,respectively,while the susceptibility of tigecycline to Acinetobacter baumannii is 91.3％ by microdilution method when choosing Oxoid MHBroth medium.Conclusion Compared with the results of other MHA mediums,using Oxoid MHB + 1.5％ Agar medium by disk diffusion method can provide a more similar result to those of the broth dilution method when test the activity of tigecycline against Acinetobacter baumannii in vitro.