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为了研究白细胞对偏振光的散射特性,运用几何光学近似理论修正偏振光传输的斯托克斯-穆勒矩阵元,基于偏心球模型数值模拟了两束偏振化方向互相垂直的偏振光入射时,不同形状、大小、折射率的无粒白细胞的散射光强空间分布以及散射特性与细胞结构的关系。不同条件下偏振光散射三维分布图像呈现不同的条纹特征,表明分布规律与细胞模型的形体参量和光学参量具有相关性。散射光强的差值、比值、差和比的分析结果表明,偏振光的后向散射反映了细胞内部更丰富的结构信息和光学信息。
In order to study the scattering characteristics of leukocytes to polarized light, the Stokes-Muller matrix element of polarized light transmission is modified by the theory of geometrical optics. When two polarized lights perpendicular to each other are incident to each other based on the eccentric spherical model, The spatial distribution of scattered light intensity of non-white blood cells with different shapes, sizes and refractive indices as well as the relationship between scattering properties and cell structure. The three-dimensional distribution images of polarized light scattering show different fringe characteristics under different conditions, indicating that the distribution rule has a correlation with the body parameters and optical parameters of the cell model. The analysis of the difference, ratio, difference and ratio of scattered light intensity shows that the backscattering of polarized light reflects the richer structural and optical information inside the cell.