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本研究以转At CBF3基因苹果嘎拉组培苗为试验材料,采用不同浓度的Na Cl为胁迫因子,探讨外源基因导入对苹果组培苗耐盐性的影响。研究结果表明:转基因苹果组培苗受害指数随着盐浓度的升高而增大,生根率随Na Cl浓度的升高而降低。在4 g/L Na Cl胁迫下,随胁迫时间的延长组培苗的受害指数呈上升趋势,在胁迫8 d时,转基因株系的受害指数显著低于非转基因株系。Na Cl胁迫明显抑制苹果组培苗不定根发生,转基因株系与非转基因株系间差异不显著。Na Cl胁迫下转基因株系的相对电导率显著低于非转基因株系。研究结果说明外源At CBF3基因的导入一定程度上提高了苹果组培苗的耐盐性,然而对其不定根发生未产生显著影响。
In this study, the At CBF3 gene Gala apple (Triticum aestivum L.) tissue culture was used as experimental material, and different concentrations of NaCl were used as stress factors to study the effects of exogenous gene introduction on the salt tolerance of apple plantlets. The results showed that the damage index of transgenic plantlets increased with the increase of salt concentration, and the rooting rate decreased with the increase of NaCl concentration. Under 4 g / L NaCl stress, the damage index of tissue culture seedlings increased with the prolonging of stress time. The damage index of transgenic lines was significantly lower than that of non-transgenic lines at 8 d. Na stress significantly inhibited the adventitious root formation of apple plantlets, and the difference between transgenic lines and non-transgenic lines was not significant. The relative conductivity of transgenic lines under NaCl stress was significantly lower than that of non-transgenic lines. The results showed that the introduction of exogenous At CBF3 gene increased the salt tolerance of apple plantlets to some extent, however, no significant effect on adventitious root formation was observed.