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目的:初步观察血管内皮祖细胞参与人结肠癌肺转移灶血管生成的情况。方法:体外传代扩增血管内皮祖细胞(EPC)后,采用4’6-二脒基-2-苯基吲哚盐酸盐(DAPI)标记,由尾静脉注入接种人结肠腺癌细胞株LS-174-T细胞7用的荷瘤裸鼠体内,分别于接种1周、2周后处死动物,摘取肺脏冰冻切片,荧光显微镜下观察血管内皮祖细胞参与结肠癌肺转移灶血管形成。结果:荧光显微镜下可以观察,接种血管内皮祖细胞1周后的裸鼠肺部血管中有发出蓝色荧光的血管内皮祖细胞,接种2用后肺部转移灶中有蓝色荧光。结论:血管内皮祖细胞可参与人结肠癌肺转移灶血管形成。
OBJECTIVE: To observe the angiogenesis of colon cancer lung metastases by vascular endothelial progenitor cells. Methods: EPCs were passaged in vitro and labeled with 4’6-diamidino-2-phenylindole hydrochloride (DAPI). Human colon adenocarcinoma cell line LS -174-T cells 7 in nude mice were sacrificed at 1 week and 2 weeks after inoculation. The frozen sections of the lungs were harvested and the endothelial progenitor cells (VECs) were observed under a fluorescence microscope to participate in the formation of colon cancer lung metastases. Results: Fluorescence microscopy showed that after one week of inoculation of vascular endothelial progenitor cells, blue fluorescence of vascular endothelial progenitor cells were found in the lung vessels of nude mice, and blue fluorescence was found in lung metastases after inoculation. Conclusion: Vascular endothelial progenitor cells can participate in the formation of colon cancer lung metastases.