在短暂性局灶脑缺血模型上，以依赖还原型辅酶Ⅱ的黄递酶（ＮＡＤＰＨ－ｄ）作为一氧化氮合酶（ＮＯＳ）的代表物，用组织化学染色方法结合计算机图像分析，观察局部神经细胞在遭受缺血缺氧后，ＮＯＳ早期变化情况。结果表明，缺血侧早期ＮＯＳ含量和活性与非缺血侧相比明显升高，ＮＯＳ含量和活性与缺血时间呈负相关线性关系。结合模型上的组织学变化，显示生物酶学变化在前，形态学改变滞后的规律。 In the transient focal cerebral ischemia model, the diaphorase (NADPH-d), which is dependent on reduced coenzyme Ⅱ, was used as the representative of nitric oxide synthase (NOS). Histochemical staining and computer image analysis were performed to observe Local nerve cells suffer from ischemia and hypoxia, the early changes in NOS. The results showed that NOS content and activity in early ischemic phase were significantly increased compared with those in non-ischemic side, and there was a negative correlation between NOS content and activity and ischemic time. Combined with the histological changes on the model, it shows that the change of the bio-enzymology is in the former and the morphological change lags behind.