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用一步法微囊技术包裹新生猪胰岛 ,并控制微囊内胰岛数量 ,分别移植于链脲霉素 (STZ)制备的糖尿病模型大鼠 ,每组 10只 ,对照组微囊内 3～ 4个胰岛 ,实验组微囊内含一个胰岛。植入胰岛数量均为2 0 0 0个 /鼠 ,移植后的第 10天各组大鼠均停用胰岛素。结果表明 :实验组大鼠血糖值为 (6 .6 4± 1.10 )mmol/L。半数以上血糖在正常范围并存活达 147d以上。对照组为 (9.83± 1.33)mmol/L。血糖维持该水平70余天后逐渐上升 ,其中 3只血糖高水平情况下存活 98d。两组相比有非常显著差异 (P <0 .0 1)。结果表明 :减少微囊内胰岛数量 ,可提供足量营养 ,是提高疗效的有利措施。
One-step microencapsulation technique was used to encapsulate newborn pig islets and to control the amount of pancreatic islets in the microcapsules. The rats were respectively transplanted into diabetic rats with streptozotocin (STZ), 10 in each group and 3 to 4 in the control group Islet, the experimental group of microcapsules containing a islet. The number of islets implanted was 200 / mouse. On the 10th day after transplantation, insulin was withdrawn from each group of rats. The results showed that the blood glucose of the experimental group was (6.64 ± 1.10) mmol / L. More than half of the blood glucose in the normal range and survival of more than 147d. The control group was (9.83 ± 1.33) mmol / L. Blood glucose maintained the level of more than 70 days after the gradual rise, of which three cases of high blood sugar survival 98d. There was a significant difference between the two groups (P <0.01). The results showed that reducing the number of islets in the microcapsule could provide adequate nutrition and was an advantageous measure to improve the curative effect.