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目的观察鹿蹄草总黄酮(tolal flavones of Herba Pyrdae,TFHP)抑制病理性动脉内膜增生,并初步探讨其作用机制。方法采用小鼠颈总动脉结扎所致内膜增生和管腔狭窄模型和血管平滑肌细胞(VSMCs)增殖试验(MTT法)。结果ICR小鼠颈总动脉结扎28 d成功复制出内膜增生和管腔狭窄,TFHP具抑制作用。HE染色切片图像分析显示,TFHP 50 mg/kg组颈总动脉管腔面积、内膜面积、中膜面积、相对管腔面积与模型组比较均无差异(P>0.05),但内膜/中膜面积比值明显减小(P<0.01);TFHP 100、200 mg/kg组内膜面积、内膜/中膜面积比值均较模型组减小(P<0.05,0.01),管腔面积、相对管腔面积均较模型组增大(P<0.01)。免疫组化染色显示,与模型组相比,TFHP各组血管壁增殖细胞核抗原阳性细胞指数均减少(P<0.01)。MTT法研究表明,小鼠颈总动脉平滑肌细胞培养48 h后,TFHP各组细胞增殖活性随TFHP浓度增加而减小,与对照组比较,均有显著差异(P<0.05,0.01)。结论TEHP能抑制病理性动脉内膜增生和管腔狭窄,可能与抑制VSMCs增殖有关。
Objective To observe the inhibition of pathological arterial intimal hyperplasia by tolal flavones of Herba Pyrdae (TFHP) and to explore its mechanism. Methods The model of intimal hyperplasia and luminal stenosis induced by ligation of common carotid arteries in mice and proliferation of vascular smooth muscle cells (VSMCs) (MTT method) were used. Results The intimal hyperplasia and luminal stenosis were successfully reproduced in the ICAR mouse carotid artery ligation for 28 days. TFHP had inhibitory effect. The image analysis of HE staining showed that the luminal area, intima area, medial area and relative lumen area of the common carotid artery in the TFHP 50 mg/kg group were not significantly different from those in the model group (P>0.05), but the intima/media The ratio of membrane area was significantly reduced (P<0.01); the ratio of intima area and intima/media area of TFHP 100 and 200 mg/kg group were lower than those of model group (P<0.05, 0.01), lumen area, relative The lumen area was larger than that of the model group (P<0.01). Immunohistochemical staining showed that compared with the model group, the positive cell index of proliferating cell nuclear antigen in the vascular wall of each group was decreased (P<0.01). MTT assay showed that after cultured for 48 h in mouse common carotid artery smooth muscle cells, the proliferation activity of TFHP in each group decreased with the increase of TFHP concentration, and there was a significant difference compared with the control group (P<0.05, 0.01). Conclusion TEHP can inhibit pathological arterial intimal hyperplasia and luminal stenosis, which may be related to the inhibition of proliferation of VSMCs.