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目的 :研究妇人 ,母猪 ,雌性大白鼠食用后 ,尿中大豆异黄酮代谢物成份的比较。方法 :妇人食用大豆食品或以含大豆饲料喂食母猪 ,雌性大白鼠后 ,收集尿液 ,以液质联用分析仪分析尿液中大豆异黄酮代谢物的种类及含量。结果 :在尿液样品中检测出大豆异黄酮及其糖苷酸代谢物。在大白鼠尿液中的大豆异黄酮代谢物主要为 7 4’ 异黄烷二醇葡萄糖苷酸 (equolglucuronideconjugate) ,其次为 7,5 ,4’ 三羟基异黄酮糖苷酸 (genisteinglucuronideconjugate)及 7,4’ 二羟基异黄酮糖苷酸 (daidzeinglucuronideconjugate)。在妇人尿液中大豆异黄酮糖苷酸代谢物超过 90 % ,在母猪尿液中大豆异黄酮糖苷酸代谢物介于 80 .5 %～ 84 .5 %之间。在雌性大白鼠尿液中大豆异黄酮糖苷酸代谢物约有 5 0 %的大豆异黄酮糖苷酸代谢物。结论 :7 4’ 异黄烷二醇为大白鼠尿液中的主要大豆异黄酮代谢物。猪模型比大白鼠模型较合适用来模拟人类食用大豆食品后的代谢研究
OBJECTIVE: To study the comparison of urinary soy isoflavone metabolites after ingestion of women, sows and female rats. Methods: After the woman consumed the soybean food or fed the soybean containing feed to the female and female rats, the urine was collected and the type and content of the metabolite of soybean isoflavone in urine were analyzed by LC / MS. Results: Soy isoflavones and their glycoside metabolites were detected in urine samples. The metabolites of soy isoflavones in urine of rats are primarily 74 ’isoolglucuronideconjugate, followed by 7,5,4’ genisteinglucuronideconjugate and 7,4 ’Daidzeinglucuronideconjugate.’ Soy isoflavone glucuronide metabolites exceeded 90% in the urine of a woman, and soy wasoflavone glucuronide metabolites ranged from 80.5% to 84.5% in sow urine. Soy isoflavone glucuronide metabolites are about 50% soy isoflavone glucuronide metabolites in the urine of female rats. CONCLUSION: 7 4 ’isoflavone diol is the major soy isoflavone metabolite in urine of rats. Pig model is more suitable than the rat model to simulate the metabolism of human consumption of soy foods