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目的通过对当归根际土壤提取液的生物学试验和有机化合物的分离鉴定,探讨当归连作障碍与自毒作用的关系。方法设置水(CK)、当归根际土水提液100、125、250、500 mg/mL共5个处理,用于当归自毒作用的生物学实验;气相色谱-质谱联用技术(GC-MS)鉴定当归根际土壤有机化合物。结果125 mg/mL当归根际土壤水提液能显著抑制当归自身种子发芽率、发芽指数及胚根和胚芽的伸长,且随水提液浓度的升高,这种抑制作用增强。在当归根际土壤水提液中鉴定到17个化合物,包括有机酸、酮、醛、酯和烃类等化感物质,其中很多被报道是化感物质。结论当归根际土壤水提液对自身种子萌发和幼苗生长具有明显的自毒作用,且自毒效应具有一定的浓度依赖性,推断自毒作用可能是造成当归连作障碍的原因之一。
OBJECTIVE: To investigate the relationship between angelica continuous cropping obstacle and autotoxicity through biological experiments and isolation and identification of organic compounds in rhizosphere soil of Angelica sinensis. Methods A total of 5 treatments including water (CK), angelica rhizosphere soil water extract of 100, 125, 250, and 500 mg / mL were used for the biological experiments of Angelica sinensis autotoxicity. Gas chromatography-mass spectrometry (GC- MS) to identify rhizosphere soil organic compounds. Results The aqueous extract of 125 mg / mL rhizosphere soil could significantly inhibit the germination rate, germination index and the elongation of radicle and germ in Angelica sinensis. With the increase of water extract concentration, the inhibitory effect was enhanced. Seventeen compounds, including organic acids, ketones, aldehydes, esters and hydrocarbons, were identified in the angelica rhizosphere soil water extract. Many of them were reported as allelochemicals. Conclusion Angelica sinensis rhizosphere soil water extract on its own seed germination and seedling growth has obvious autotoxicity, and the autotoxic effect has a certain concentration-dependent, infer the role of autotoxins may be caused by Angelica continuous cropping obstacles one of the reasons.