遗传工程重组蛋白质N-端序列和C-端序列分析是鉴定其结构特征的2项重要的指标.N-端的序列分析即采用经典的Edman方法,在商品化的仪器上自动分析50个以内的N-端氨基酸残基,已经是一种常规方法,近年来技术发展可以通过SDS-PAGE电泳印迹到PVDF膜上,在膜上直接分析蛋白的N-端序列,不但可以用来测定产品的顺序,而且为检验基因工程培养物是否与预期表达产物相吻合提供了一个相当简便的方法. N-terminal sequence and C-terminal sequence analysis of genetically engineered recombinant proteins are two important indexes to identify their structural features.The N-terminal sequence analysis is based on the classical Edman method and automatically analyzes up to 50 N-terminal amino acid residues, is already a conventional method, in recent years, technological development can be blotted to PVDF membrane by SDS-PAGE electrophoresis, direct analysis of the N-terminal sequence of the protein membrane, not only can be used to determine the order of the products , But also provides a fairly easy way to test whether the engineered culture conforms to the expected expression product.