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目的对绵萆薢的化学成分进行研究。方法将大孔吸附树脂、硅胶、Sephadex LH-20、正相硅胶、ODS等柱色谱与制备型高效液相色谱法相结合进行化合物的分离制备,并通过理化性质与核磁共振波谱数据鉴定化合物的结构。结果从绵萆薢中分离鉴定了12个单体成分,分别鉴定为原薯蓣皂苷(protodioscin,1)、甲基原薯蓣皂苷(methyl protodioscin,2)、原纤细皂苷(protogracillin,3)、甲基原纤细皂苷(methyl protogracillin,4)、伪原薯蓣皂苷(pseudoprotodioscin,5)、伪原纤细皂苷(pseudoprotogracillin,6)、26-O-β-D-glucopyranosyl-3β,23,26-triol-25(R)-furosta-5,20(22)-dien-3-O-α-L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside(7)、豆甾醇(stigmasterol,8)、β-谷甾醇(β-sitosterol,9)dioscoreavilloside A(10)、绵萆薢苷B(spongioside B,11)及粉背薯蓣G(hypoglaucin G,12)。结论在对绵萆薢的化学成分进行研究的过程中,分离鉴定了12个甾体类化合物。其中化合物5~8和10为首次从该种植物中分离得到,并首次对化合物7的核磁数据进行了报道。
Objective To study the chemical composition of cottontail Methods The compounds were separated and synthesized by macroporous adsorption resin, silica gel, Sephadex LH-20, normal phase silica gel and ODS column chromatography and preparative HPLC. The structures of the compounds were identified by physico-chemical and nuclear magnetic resonance spectroscopy . RESULTS: Twelve monomer components were isolated and identified from Protocamellia japonica. They were identified as protodioscin (1), methyl protodioscin (2), protogracillin (3) Pseudoprotodioscin (5), pseudoprotogracillin (6), 26-O-β-D-glucopyranosyl-3β, 23,26-triol-25 -furosta-5,20 (22) -dien-3-O-α-L-rhamnopyranosyl (1 → 4) - [α-L-rhamnopyranosyl- , Stigmasterol (8), β-sitosterol (9) dioscoreavilloside A (10), spongioside B (11) and hypoglaucin G (12). Conclusion In the study on the chemical constituents of Gynostemma, the 12 steroidal compounds were isolated and identified. Among them, compounds 5 ~ 8 and 10 were isolated from this plant for the first time and the NMR data of compound 7 were reported for the first time.