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目的:探讨STAT3及其下游基因CyclinD1在子宫肌瘤的表达和意义。方法:采用RT-PCR方法检测子宫肌瘤和同源正常子宫平滑肌组织中STAT3及其下游基因CyclinD1的表达,采用免疫组化方法检测子宫肌瘤和同源正常子宫平滑肌组织中磷酸化STAT3的表达。结果:STAT3mRNA在子宫肌瘤表达明显高于正常的子宫平滑肌组织,P<0.01;CyclinD1mRNA在子宫肌瘤的表达也相应升高。磷酸化STAT3在子宫肌瘤和同源正常子宫平滑肌的阳性表达率分别是77.8%和5.56%,差异有统计学意义(P<0.01)。结论:子宫肌瘤STAT3及CyclinD1表达增高,STAT3和CyclinD1在子宫肌瘤的发病中起了一定作用。
Objective: To investigate the expression and significance of STAT3 and its downstream gene CyclinD1 in uterine fibroids. Methods: The expression of STAT3 and its downstream gene CyclinD1 in uterine fibroids and homologous normal uterine smooth muscle tissues were detected by RT-PCR. The expression of phosphorylated STAT3 in uterine fibroids and normal uterine smooth muscle tissues was detected by immunohistochemistry . Results: STAT3 mRNA expression in uterine fibroids was significantly higher than normal uterine smooth muscle tissue, P <0.01; CyclinD1 mRNA expression in uterine fibroids also increased accordingly. The positive rates of phosphorylated STAT3 in uterine fibroids and homologous normal uterine smooth muscle were 77.8% and 5.56% respectively, with statistical significance (P <0.01). Conclusion: The expression of STAT3 and CyclinD1 in uterine fibroids is increased, and STAT3 and CyclinD1 play a role in the pathogenesis of uterine fibroids.