采用高效液相色谱法测定氟康唑软膏的含量。用Ｓｐｈｅｒｉｓｏｒｂ － Ｃ１８ 色谱柱， 甲醇－ 水（５０∶５０） 为流动相， 检测波长为２６１ｎｍ ， 用外标法计算含量。理论塔板数按氟康唑峰计算约为３０００ 。以峰面积计算氟康唑在５０－１２ ～５０１－２μｇ／ｍｌ 浓度范围内呈线性关系， ｒ＝ ０－９９９９ 。平均回收率为９９－６１ ％ （ｎ ＝ ５） ， ＲＳＤ＝ ０－４９％ 。方法准确， 简便， 专属性强。 Determination of fluconazole ointment by high performance liquid chromatography. Using Spherisorb - C18 column, methanol - water (50:50) as the mobile phase, the detection wavelength was 261 nm and the content was calculated by external standard method. The number of theoretical plates calculated by fluconazole peak is about 3000. Fluconazole peak area in the 50-12 ~ 501-2μg / ml concentration range of a linear relationship, r = 0-9999. The average recovery was 99-61% (n = 5), RSD = 0-49%. The method is accurate, simple and specific.