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目的探讨原因不明复发性流产患者中Foxp3和VEGF基因、HLA-G基因及NTHFR基因多态性。方法利用聚合酶链反应和基因测序的方法,对50例复发性流产患者(流产组)与10例可孕妇女(对照组)Foxp3基因和VEGF、HLA-G、MTHFR基因的外显子测序。结果研究中检测出4个基因的多种突变,流产组rs1801133 TT基因型频率只在流产组中出现,rs1801131的基因型频率和等位基因频率分布在两组间差异无统计学意义(P>0.05)。结论我们的研究结论表明rs1801133 TT基因型可能与不良妊娠相关。
Objective To investigate the polymorphisms of Foxp3, VEGF, HLA-G and NTHFR in patients with unexplained recurrent spontaneous abortion. Methods The exons of Foxp3, VEGF, HLA-G and MTHFR genes in 50 recurrent spontaneous abortion patients (abortion group) and 10 pregnant women (control group) were sequenced by polymerase chain reaction and gene sequencing. Results A variety of four mutations were detected in the study. The genotype frequency of rs1801133 TT in abortion group only appeared in abortion group. The genotype frequency and allele frequency distribution of rs1801131 in two groups showed no significant difference (P> 0.05). Conclusions Our findings suggest that rs1801133 TT genotype may be associated with adverse pregnancy.