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目的 :研究新近报道的一种与输血后肝炎有关的病毒在中国郑州地区正常人群、HBsAg阳性者、非甲至非戊型肝炎患者中的感染和基因序列变异情况。方法 :采用TTV基因组ORF1区的套式聚合酶链反应 (nested PCR)方法对血清标本进行检测 ,并对非甲至非戊型肝炎患者中的TTV分离株进行序列测定。结果 :TTVDNA在非甲至非戊型肝炎患者中检出率为 45 % (1 8/ 40 ) ;在正常体检者中检出率为 1 7.7% (1 7/ 96 ) ;在HBsAg阳性者 (ALT≤ 34IU/L)中检出率 2 2 .7% (1 0 / 44 )。TTV分离株序列与日本株 (CLON2 2 )相对应位置的核苷酸同源性为 99.1 %。结论 :TTV在非甲至非戊型肝炎患者TTV的感染率明显高于其他人群 ,可能是非甲至非戊型肝炎的主要致病因子。
Objective: To study the recent infection and gene sequence variation of a post-transfusion hepatitis-related virus in normal population, HBsAg-positive and non-A-to-E hepatitis patients in Zhengzhou, China. Methods: Serum samples were detected by nested PCR using the ORF1 region of TTV genome. The TTV isolates from non-A to non-E hepatitis patients were sequenced. Results: The detection rate of TTVDNA was 45% (18/40) in patients with non-A and non-E hepatitis; the detection rate was 1 7.7% (17/96) in normal subjects; in HBsAg-positive patients ALT ≤ 34IU / L) detection rate of 22.7% (10/44). The nucleotide homology between the TTV isolates and the Japanese strain (CLON2 2) was 99.1%. Conclusion: The prevalence of TTV in patients with non-A and non-E hepatitis is significantly higher than that in other populations and may be the main causative agent of non-A and non-E hepatitis.