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目的 :研究牙龈卟啉单胞菌定植对钛表面氧化膜特性的影响。方法 :将纯钛试件浸泡于牙龈卟啉单胞菌菌悬液中进行共培养,采用扫描电镜观察钛表面牙龈卟啉单胞菌生物膜的微结构。以牙龈卟啉单胞菌菌悬液为实验组,生理盐水为对照组,收集7、14、21 d的试件和浸泡液,采用X射线光电子能谱(XPS)分析浸泡后纯钛的表面特性,采用电感耦合-等离子体发射光谱仪(ICP-OES)检测不同时间点浸泡液中的钛离子释放量。结果:扫描电镜观察到牙龈卟啉单胞菌在钛表面定植形成致密的生物膜。XPS广谱分析显示,钛表面的钛和氧元素含量随细菌作用时间延长逐步降低;XPS高像素谱分析显示,钛表面的二氧化钛含量随细菌作用时间延长逐步降低。ICP-OES检测发现随着细菌作用时间的延长,钛离子释放量增加,且第1周释放量最多。结论:牙龈卟啉单胞菌能在钛表面定植形成致密生物膜,引发钛表面氧化膜破坏和钛离子释放,该效应随细菌作用时间延长而加剧。
Objective: To study the effect of Porphyromonas gingivalis on the surface oxide film properties of titanium. Methods: Pure titanium specimens were immersed in suspension of Porphyromonas gingivalis for co-culture, and the microstructure of Porphyromonas gingivalis biofilm on titanium surface was observed by scanning electron microscopy. Porphyromonas gingivalis suspension was used as experimental group and normal saline as control group. The specimens and immersion liquid were collected for 7, 14 and 21 days. X-ray photoelectron spectroscopy (XPS) was used to analyze the surface of pure titanium The ICP-OES was used to detect the release of titanium ions in the soaking solution at different time points. Results: Scanning electron microscopy showed that Porphyromonas gingivalis colonized on titanium surface to form a dense biofilm. XPS spectrum analysis showed that the content of titanium and oxygen on the surface of titanium gradually decreased with the prolongation of bacterial action time. XPS high resolution spectral analysis showed that the content of titanium dioxide on the surface of titanium decreased gradually with the prolongation of bacterial action. ICP-OES assay showed that with the extension of bacterial action time, the release of titanium ions increased, and the release amount of the first week was the highest. Conclusion: Porphyromonas gingivalis can colonize titanium surface to form dense biofilm, which can cause the destruction of titanium oxide film and the release of titanium ions. This effect aggravates with the prolongation of bacterial action time.