建立环维黄杨星D磷脂复合物复合率的间接测定方法。采用紫外分光光度法测定未参与复合的环维黄杨星D的量,由此计算复合率。测定波长为410nm,结果表明,浓度在0.502—2.510μg/mL时与吸光度呈良好的线性关系,回归方程为:A=0.1805C+0.0666,r=0.9993(n=5);平均回收率为99.73%,RSD为1.78%。本方法操作简便易行,具有良好的重现性和精密度。 Indirect determination of compounding rate of cyclophosphamide D phospholipid complex. UV spectrophotometry was used to determine the amount of cyclovirobuxine D that did not participate in the complex, thus the recombination rate was calculated. The determination wavelength was 410nm, the results showed that the concentration of 0.502-2.510μg / mL and absorbance showed a good linear relationship, the regression equation was: A = 0.1805C +0.0666, r = 0.9993 (n = 5); The average recovery was 99.73 %, RSD is 1.78%. The method is simple and easy to operate with good reproducibility and precision.