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目的:建立HPLC测定武打将军酒中大黄素含量的方法。方法:色谱柱为Phenomenex Genini C_(18)(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸溶液(70:30),检测波长为254 nm,流速为1.0 ml·min~(-1)。结果:大黄素在5.045～50.450μg·ml~(-1)范围内线性关系良好(r=0.999 9),平均加样回收率为99.85%,RSD为1.0%(n=6)。结论:方法简便、准确、重复性好,可用于武打将军酒中大黄素的质量控制。
Objective: To establish a HPLC method for determination of emodin in wushu wine. METHODS: Phenomenex Genini C_ (18) (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase was methanol-0.1% phosphoric acid solution (70:30) at a detection wavelength of 254 nm with a flow rate of 1.0 ml · min ~ -1). Results: The emodin showed good linearity (r = 0.999 9) in the range of 5.045-50.450 μg · ml -1 with an average recovery of 99.85% and a RSD of 1.0% (n = 6). Conclusion: The method is simple, accurate and reproducible. It can be used for the quality control of emodin in wine.