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在温室条件下,通过高效液相色谱法,测定了不同刈割高度(2.5,5.0和7.5cm)和刈割频度(1周1次和2周1次)对醉马草甘肃内生真菌共生体产生麦角酰胺和麦角新碱量的影响.前4个月醉马草中生物碱的含量基本成线性增加,在不同刈割高度处理中,7.5cm的刈割高度条件下生物碱含量要高于2.5cm的刈割高度;在刈割频度处理中,两周刈割一次生物碱含量要高于1周刈割1次.实验过程中检测到生物碱含量最高值可达最低值的2～3倍.上述结果表明,如果将醉马草内生真菌应用于植物保护,必须意识到潜在的植物管理因素(刈割频率或者播种密度等)的实施可能会降低或者提高植物保护的水平,相同的管理因素也可能影响其他禾草-内生真菌共生体中生物碱的含量,所以管理因素对禾草-内生真菌共生体产碱的影响将会是未来一个非常有趣的话题.
Endophytic fungi were isolated from Gansu, China by high performance liquid chromatography (HPLC) at different harvesting heights (2.5, 5.0 and 7.5 cm) and mowing frequency (once weekly and once weekly) Symbiont produce ergamil and ergometrine quantity.The content of alkaloids in drunken horsegrass basically increased linearly in the first 4 months and the content of alkaloids in the cutting height of 7.5cm Higher than the cutting height of 2.5cm; cutting frequency treatment, cutting two weeks once alkaloid content is higher than 1 week mowing 1. The maximum alkaloid content was detected during the experiment up to the lowest value 2 to 3. The above results indicate that if B. drubberi endophyte is applied to plant protection, it must be realized that the implementation of potential plant management factors (cutting frequency or sowing density, etc.) may reduce or increase the level of plant protection , The same management factors may also affect the alkaloid content of other grass-endophytic fungal symbionts, so the impact of management factors on the alkali-producing of the grass-endophyte symbiont will be a very interesting topic in the future.