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制备了硅溶胶前驱体硅酸甘油二酯(diglycerol silicate,DGS),利用动态光散射跟踪法考察了其水解和凝胶过程。结果表明:所制备的DGS纯度较高,水解后形成的硅溶胶粒子zeta电位为-12.56mV。离子强度、蛋白种类及浓度均对硅溶胶-凝胶体系的胶凝时间和凝胶状态有影响。在凝胶温度为30℃,DGS质量分数为10%,离子浓度为2mmol/L,蛋白质量分数为0.13%时,可获得均匀性较好的凝胶体系,该体系凝胶时间为30min。DGS水解产物的自相关函数变化和散射光强变化均为典型硅凝胶体系的变化。该体系可作为维持生物大分子活性的理想体系。
The silica sol precursor diglycerol silicate (DGS) was prepared and its hydrolysis and gelation process were investigated by dynamic light scattering (DLS) method. The results showed that the purity of DGS prepared was high, and the zeta potential of silica sol formed after hydrolysis was -12.56mV. The ionic strength, protein type and concentration all influence the gel time and gel state of silica sol-gel system. When the gel temperature was 30 ℃, the mass fraction of DGS was 10%, the ion concentration was 2mmol / L and the protein content was 0.13%, a better homogeneous gel system was obtained. The gel time was 30min. The changes of autocorrelation function and scattering intensity of DGS hydrolyzate are the changes of typical silica gel system. The system can be used as an ideal system to maintain the biological activity of macromolecules.