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用正常成年豚鼠20只、大鼠10只,颈椎脱臼或重击头部处死后,立即取胸腺、淋巴结和小肠,部分材料投入-70℃液氮速冻,部分材料入10%甲醛和纯甲醇固定,石蜡切片,HE和肥大细胞特殊染色;肠系膜铺片经氮气吹干后,与液氮速冻的组织块一并置-70℃低温冰箱保存。可随时取出做恒冷箱切片,部分冰冻切片及肠系膜铺片用Janszen和Nugteren前列腺素合成酶组织化学法显示,以此法同步显示家兔肾脏。每种切片均用酶的特异抑制剂消炎痛作对照。部分连续冰冻切片,做HE和肥大细胞特殊染色。结果表明,前列腺素合成酶活性存在于肥大细胞的胞质内,颗粒和胞核为阴性。家兔肾脏集合管恒为阳性。消炎痛抑制反应极弱。确证前列腺素合成酶的组织化学方法可靠并且具特异性。前列腺素合成酶阳性反应的细胞,见于大鼠和豚鼠的小肠粘膜及粘膜下层、胸腺被膜和胸腺隔的结缔组织以及胸腺髓质、淋巴结被膜和小梁结缔组织及深皮质区;散布于肠系膜铺片上的细胞多呈椭圆形。以上所示酶活性细胞的分布,与肥大细胞的其他特殊染色结果相符。证明酶反应确是在肥大细胞内。本文用组织化学技术,证实肥大细胞内存在前列腺素合成酶,此酶是衡量前列腺素合成的敏感指标,可考虑用此方法研究肥大细胞的生理和病理。
Twenty normal guinea pigs and 10 rats were sacrificed and the thymus, lymph nodes and small intestine were removed immediately after the cervical vertebra was dislocated or swamped head. Some of the materials were frozen in liquid nitrogen at -70 ℃ and some of the materials were fixed with 10% formaldehyde and pure methanol , Paraffin sections, HE and mast cells were specially stained; mesenteric membrane pieces were blown dry with nitrogen and stored in a low-temperature refrigerator at -70 ℃ together with quick-frozen liquid nitrogen tissue. Can be removed at any time do constant cold box section, some frozen sections and mesenteric membrane with Janszen and Nugteren prostaglandin synthase histochemistry showed that this method simultaneously shows the kidneys of rabbits. Each slice with a specific inhibitor of indomethacin as a control. Part of the frozen section, do HE and mast cells special staining. The results showed that prostaglandin synthase activity was present in the cytoplasm of mast cells, with negative granules and nuclei. Rabbit kidney collection tube is always positive. Indomethacin inhibition is very weak. Histochemical methods to confirm prostaglandin synthase are reliable and specific. Prostaglandin synthase positive cells, found in rat and guinea pig intestinal mucosa and submucosa, thymus envelope and thymus connective tissue and thymus medulla, lymph node capsule and trabecular connective tissue and the deep cortex; scattered in the mesenteric On-chip cells were mostly oval. The distribution of the enzymatically active cells shown above is consistent with other special staining results of mast cells. Prove enzyme reaction is indeed in mast cells. In this paper, the use of histochemical techniques to confirm the presence of prostaglandin synthase in mast cells, this enzyme is a sensitive indicator of prostaglandin synthesis can be considered using this method to study mast cell physiology and pathology.