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目的 :探讨一氧化氮合酶 ( NOS)在人肝癌发生过程中的表达及作用机制。方法 :以病理组织学和分子生物学方法 ,分析16例自身配对的肝癌、癌旁组织和远癌组织中总 RNA浓度 ,并以重氮法和硝酸还原酶法分别定量不同组织中 NOS的比活性和一氧化氮 ( NO)浓度。结果 :经病理组织学证实的 16例肝癌、癌旁和远癌组织中 :1从癌灶→癌旁→远癌组织中 ,总 RNA浓度呈逐步升高趋势 ,三种不同组织间的差异均有显著性 ( P<0 .0 5 ) ;2癌灶、癌旁、远癌三种组织中 NOS比活性持续上升 ;( 3 )肝癌组织中 NOS比活性改变与肝总 RNA浓度、或与 NO水平呈明显正相关 ,其相关系数分别为 γ=0 .5 8( P<0 .0 1)和 γ=0 .47( P<0 .0 5 )。结论 :资料提示 NOS活性的异常改变 ,在肝细胞癌变过程中可能起保护作用
Objective : To investigate the expression and mechanism of nitric oxide synthase (NOS) in the development of human liver cancer. METHODS: Total RNA concentrations in 16 self-matched hepatocellular carcinomas, paracancerous tissues, and distal cancer tissues were analyzed by histopathology and molecular biology methods. The ratios of NOS in different tissues were quantified by diazo method and nitrate reductase method, respectively. Active and nitric oxide (NO) concentrations. RESULTS: Among the 16 cases of hepatocellular carcinoma, paracancerous and distant cancer tissues confirmed by histopathology: 1 The total RNA concentration was gradually increased from the lesion to the paratumor to the distal carcinoma, and the differences among the three different tissues were Significant (P<0.05); 2 NOS activity in cancer tissue, paraneoplastic, and distant cancer tissues continued to increase; (3) NOS activity in hepatocellular carcinoma changes with total liver RNA concentration, or with NO The levels were positively correlated, and the correlation coefficients were γ=0.58 (P<0.01) and γ=0.47 (P<0.05). Conclusion : The data suggest that abnormal changes in NOS activity may play a protective role in hepatocellular carcinogenesis