脐血移植已被广泛地用于治疗各种血液性疾病和恶性肿瘤 ,临床上主要采用同胞脐血移植和无关供者脐血移植两种形式[1] 。前者往往直接将整份脐血冻存 ,后者供者来源于脐血库 ,多为经羟乙基淀粉 (ＨＥＳ)分离后的有核细胞(ＮＣ) [2 ] 。对于保存方法 ,单用一种冷冻保护剂即使加大 Umbilical cord blood transplantation has been widely used for the treatment of various blood diseases and malignant tumors, the clinical use of sibling cord blood transplantation and unrelated donor cord blood transplantation in two forms [1]. The former often directly to the entire umbilical cord blood cryopreservation, the latter derived from umbilical cord blood banks, mostly hydroxyethyl starch (HES) after the separation of nucleated cells (NC) [2]. For preservation methods, use a cryoprotectant alone even if increased