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目的了解广西市售鸡肉中金黄色葡萄球菌的耐药性及肠毒素基因分型情况。方法合成SEA-SEJ 9种肠毒素基因特异性引物,用PCR方法扩增84株金黄色葡萄球菌的肠毒素基因,并用毛细管仪进行电泳;采用微量肉汤稀释法进行14种临床常用抗生素的药敏试验。结果 51.2%的菌株携带肠毒素基因,主要流行的基因型是SEI(27.4%),其次为SEA(21.4%),未检出携带SEE的金黄色葡萄球菌菌株,2种以上肠毒素基因菌株携带率为46.4%(39/84),3重以上耐药菌株占72.6%(61/84),对青霉素、红霉素、四环素、氯霉素耐药率分别为95.2%、79.8%、73.8%、63.1%,所有菌株对万古霉素均敏感。结论广西市售鸡肉金黄色葡萄球菌携带多种类型的肠毒素基因,且大部分菌株呈现出一定程度的多重耐药现象,应当限制抗生素在食源性动物饲养过程中的应用,确保食品安全。
Objective To understand the resistance of Staphylococcus aureus and the genotyping of enterotoxins in Guangxi commercial chicken. Methods SEA-SEJ 9 kinds of enterotoxin gene-specific primers were synthesized, 84 strains of Staphylococcus aureus enterotoxin gene were amplified by PCR and electrophoresed by capillary instrument. A total of 14 clinically commonly used antibiotics Sensitive test. Results 51.2% of the isolates carried the enterotoxin gene. The major epidemic genotypes were SEI (27.4%), followed by SEA (21.4%). No SEE-harboring Staphylococcus aureus strains were detected. Two or more enterotoxin gene strains were carried The rates of resistance to penicillin, erythromycin, tetracycline and chloramphenicol were 95.2%, 79.8% and 73.8% respectively, with the rate of 46.4% (39/84) , 63.1%, all strains were sensitive to vancomycin. Conclusion Staphylococcus aureus on the market in Guangxi carries many types of enterotoxin genes, and most of the strains present a certain degree of multidrug resistance. The application of antibiotics in food-bearing animals should be restricted to ensure food safety.