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目的:探索化瘀祛痰复方矢志方对尿酸代谢的影响及机制。方法:以酵母粉每日15 g/kg灌胃及氧嗪酸钾每日600 mg/kg腹腔注射2周诱导SD大鼠高尿酸血症模型。设正常组、模型组、中药(矢志方)组及苯溴马隆组,给予相应干预。2周后,检测各组大鼠血清尿酸、肌酐、尿素氮、血清黄嘌呤氧化酶(XOD)活性、尿尿酸、尿β2-MG及观察肾组织病理变化。结果:与空白组比较,模型组血尿酸、24 h尿量、尿尿酸水平、24 h尿酸总排泄量和XOD活性明显升高,差异有统计学意义(P<0.01),而血尿素氮、血肌酐、尿β2-MG差异无统计学意义(P>0.05),说明该高尿酸血症模型成功建立。给药后,与模型组比较,中药组及苯溴马隆组血尿酸显著降低、24 h尿尿酸总排泄量显著增加、血清XOD活性显著降低,差异均有统计学意义(P<0.01);中药组和苯溴马隆组各指标差异均无统计学意义(P>0.05);肾组织病理显示两治疗组病变程度较模型组均减轻。结论:矢志方可以有效降低高尿酸血症模型大鼠血尿酸的水平并可促进尿尿酸的排泄,可降低血清XOD活性,可能为其降低血尿酸的机制之一。
Objective: To explore the effect and mechanism of Huazhi Qutan Compound Zhi Zhi Fang on uric acid metabolism. Methods: Hyperuricemic model of SD rats was induced by intragastric administration of 15 g / kg yeast daily and potassium oxonicoxazine 600 mg / kg daily for 2 weeks. The normal group, model group, traditional Chinese medicine (Zhizhi square) group and benzbromarone group, given the appropriate intervention. After 2 weeks, serum uric acid, creatinine, urea nitrogen, serum xanthine oxidase (XOD) activity, uric acid and urine β2-MG were detected in all groups. Results: Compared with the blank group, the levels of serum uric acid, 24 h urine, uric acid, 24 h uric acid excretion and XOD activity in model group were significantly increased Serum creatinine, urinary β2-MG was no significant difference (P> 0.05), indicating that the hyperuricemia model was successfully established. Compared with the model group, the levels of serum uric acid and the total serum uric acid excretion at 24 h were significantly decreased (P <0.01). There was no significant difference in each index between traditional Chinese medicine group and benzbromarone group (P> 0.05). The pathological changes of renal tissue showed that the lesion degree of the two treatment groups were reduced compared with the model group. Conclusion: Zhi Zhi Fang can effectively reduce the level of serum uric acid in hyperuricemic rats and promote the excretion of uric acid and reduce the serum XOD activity, which may be one of the mechanisms of lowering serum uric acid.