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目的了解PCR检测外生殖器尖锐湿疣 (CA)表面脱落细胞及疣组织液HPV6/11的效果。方法将病理确诊为外生殖器CA的92例患者随机分成两组 ,疣表面脱落细胞组48例和疣组织液组44例 ,样本于 -4℃保存 ,用HPV6/11引物进行PCR检测,并比较不同皮疹形态的HPV6/11检出情况。结果疣表面脱落细胞组与疣组织液组的HPV6/11PCR阳性率分别为87.5 %和86.4 %,两组间无显著性差异(P>0.05)。不同皮疹形态的HPV6/11检出率无显著性差异(P>0.05)。结论CA表面脱落细胞及疣组织液可用于检测外生殖器CAHPVDNA ,扩展了PCR的应用范围。前者无创伤,取材更简便。HPV6/11检出率与皮疹形态无关
Objective To understand the effect of HPV detection on exfoliative cells on the surface of condyloma acuminata condyloma acuminatum (CA) and HPV6 / 11 of wart tissue fluid. Methods Ninety-two patients with pathologically diagnosed as genital CA were randomly divided into two groups: 48 cases of exfoliative cells on the surface of the wart and 44 cases of the warty interstitial fluid group. The samples were stored at -4 ℃ and detected by PCR using HPV6 / 11 primers Rash morphology HPV6 / 11 detected. Results The positive rates of HPV6 / 11 PCR in the exfoliated cell group and the warty interstitial fluid group were 87.5% and 86.4%, respectively, with no significant difference between the two groups (P> 0.05). There was no significant difference in the detection rate of HPV6 / 11 between different rash forms (P> 0.05). Conclusion CA surface exfoliated cells and warts tissue fluid can be used to detect genital CAHPVDNA, extended the scope of PCR. The former noninvasive, drawing more convenient. HPV6 / 11 detection rate and rash morphology has nothing to do