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目的通过MGIT960培养系统结合国外最新的INNO -LIPATM RIF.TB(LIPA)DNA探针技术的应用 ,探讨其快速诊断结核病的可行性。方法应用BACTECMGIT960系统进行临床疑似结核病例标本的培养 ,筛选了70例结核分枝杆菌 ,35例非结核分枝杆菌 ,用LIPA探针技术诊断是否为结核分枝杆菌。结果用LIPADNA方法在70例结核分枝杆菌中获得阳性69例 ,占98.6 % ;35例非结核分枝杆菌均为阴性。与单纯消化纯化后涂片的82.9 % (58/70)的阳性率相比增加了15.7个百分点 ,两者在统计学上差异有显著性(P<0.05)。BactecMGIT960培养结合LIPADNA探针与传统诊断方法4～8周相比 ,整个过程只需6～37天 ,平均15天 (2周 )。结论LIPADNA探针是一种具有高度敏感性和特异性、能够简便、快速、准确地诊断结核病的新技术。同时还能鉴定rpoβ 基因位点的突变 ,诊断耐利福平 (RMP)性结核 ,有望适用于结核分枝杆菌耐RMP性结核的快速检测 ,进一步为结核病快速准确的治疗提供理论依据。
Objective To explore the feasibility of rapid diagnosis of tuberculosis by combining MGIT960 culture system with the latest foreign INNO-LIPATM RIF.TB (LIPA) DNA probe technology. Methods BACTECMGIT960 system was used to culture clinical samples of suspected TB cases. Seventy cases of Mycobacterium tuberculosis and 35 cases of non-tuberculous mycobacteria were screened out. Mycobacterium tuberculosis was diagnosed by LIPA probe technique. Results The LIPADNA method was positive in 70 cases of Mycobacterium tuberculosis in 69 cases, accounting for 98.6%; 35 cases of non-tuberculous mycobacteria were negative. Compared with the positive rate of 82.9% (58/70) in pure digestion and purification smear, the positive rate increased 15.7%, the difference was statistically significant (P <0.05). BactecMGIT960 cultured with LIPADNA probe compared with the traditional diagnostic methods 4-8 weeks compared to the entire process only 6 to 37 days, an average of 15 days (2 weeks). Conclusion The LIPADNA probe is a highly sensitive and specific technique that can diagnose tuberculosis easily, rapidly and accurately. At the same time, it can also identify mutations of rpoβ gene locus and diagnose Rifampicin (RMP) tuberculosis. It is expected to be applied to the rapid detection of Mycobacterium tuberculosis RMP-resistant tuberculosis and further provide a theoretical basis for the rapid and accurate treatment of tuberculosis.