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实验用体内、外相结合的方法，观察了Ｄ－半乳糖（Ｄ－ｇａｌａｃｔｏｓｅ，Ｄ－ｇａｌ．）对细胞的促老化作用。结果表明，Ｄ－ｇａｌ使培养大鼠胎脑神经元出现生长发育缓慢、突起脱落、死亡率增高等退行性变；大鼠肺成纤维细胞的体外分裂代数减少；人胎儿肺二倍体成纤维细胞（ＨＢＳ）分裂周期中Ｇ２－Ｍ期细胞减少、Ｇ－Ｇ１期细胞增加、ＤＮＡ含量下降，细胞增殖速度减慢；大鼠胎脑神经细胞和ＨＢＳ中ＳＯＤ含量降低，丙二醛（ＭＤＡ）水平升高，这些结果与次黄瞟呤（ＨＰＸ）－黄瞟呤氧化酶（ＸＯＤ）系统产生的超氧阴离子自由基（Ｏ－２·）作用一致。提示，Ｄ－ｇａｌ抑制细胞生长发育和分裂繁殖，加速细胞老化，其机理可能与Ｏ－２·等活性氧（ＲＯＳ）过荷引起的脂质过氧化有关。
In vitro and in vivo experiments were conducted to observe the effects of D-galactose (D-gal.) On cell growth. The results showed that D-gal induced degeneration of neurons in cultured rat fetal brain neurons, such as slow growth and development, neurite outgrowth and increased mortality; The number of G2-M phase cells decreased, the number of G-G1 phase cells increased, the DNA content decreased and the cell proliferation rate slowed down. The content of SOD in fetal rat brain neurons and HBS decreased, while malondialdehyde (MDA) These results are consistent with the role of the superoxide anion radical (O-2 ·) produced by the hypoxanthine (HPX) -xanthate oxidase (XOD) system. These results suggest that D-gal inhibits cell growth and division and accelerates cell aging. The mechanism may be related to lipid peroxidation caused by O-2 · and other reactive oxygen species (ROS) overload.