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目的探讨凋亡相关基因Bcl-2、Fas(又称Apo-1抗原,CD95)在重症肌无力患者胸腺组织中的表达及其临床意义。方法通过免疫组化法,检测经手术治疗的56例重症肌无力患者的胸腺组织(31例胸腺增生、25例胸腺瘤)中Bcl-2、Fas的表达水平,并以25例正常胸腺组织(取自先天性心脏病手术中因显露视野需要而切除的胸腺组织)作为对照组。结果Bcl-2在胸腺瘤、胸腺增生组织中的表达均高于对照组,差别有显著性意义(均P<0.01),胸腺瘤和胸腺增生两组之间的Bcl-2表达水平差别无显著性意义(P>0.05);Fas在胸腺瘤中的表达上升,明显高于胸腺增生组和对照组(均P<0.01),胸腺增生组与对照组Fas表达水平差别无显著性意义(P>0.05)。结论Bcl-2的高水平表达导致胸腺免疫异常,在重症肌无力的发生中起重要作用,而Fas在胸腺瘤的发生中起重要的作用。
Objective To investigate the expression of Bcl-2, Fas (apo-1 antigen, CD95) in thymic tissue of patients with myasthenia gravis and its clinical significance. Methods Immunohistochemistry was used to detect the expression of Bcl-2 and Fas in thymus (31 cases of thymus hyperplasia and 25 cases of thymoma) in 56 patients with myasthenia gravis surgically treated with 25 cases of normal thymus tissue Thymus tissue removed from the need to reveal visual field during congenital heart surgery) served as a control group. Results The expression of Bcl-2 in thymoma and thymus hyperplasia tissues was significantly higher than that in control group (all P <0.01). There was no significant difference in Bcl-2 expression between thymoma and thymus hyperplasia (P> 0.05). The expression of Fas in thymoma was significantly higher than that in thymus hyperplasia group and control group (all P <0.01). There was no significant difference in Fas expression between thymus hyperplasia group and control group (P> 0.05). Conclusion The high level expression of Bcl-2 leads to abnormal thymic immunity and plays an important role in the development of myasthenia gravis. Fas plays an important role in the occurrence of thymoma.