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建立原药材制首乌及复方虫草胶囊中 2 ,3 ,5 ,4′ 四羟基二苯乙烯 2 O β D 葡萄糖苷的含量测定方法。方法 :YWG C18柱 (4 .6mm× 2 5 0mm ,10 μm) ;流动相乙腈 水 (1∶4) ;检测波长 :32 0nm ,参比波长 380nm ;流速 :1ml·min-1;柱温 :室温。结果 :2 ,3 ,5 ,4′ 四羟基二苯乙烯 2 O β D 葡萄糖苷对照品在 1.6 5～ 3 .85 μg范围内 ,进样量与峰面积间呈良好的线性关系 ,r=0 .9997,平均回收率 (98.84± 0 .2 3) %。对照品在复方虫草胶囊中的峰纯度因子为 999.35 2。结论 :用HPLC法测定原药材制首乌及复方虫草胶囊中 2 ,3,5 ,4′ 四羟基葡萄糖苷的含量 ,操作简便 ,结果准确 ,可用于控制原药材制首乌及复方虫草胶囊制剂的质量。
To establish a method for the determination of 2, 3, 5, and 4′ tetrahydroxystilbene 2 O β D glucoside in Radix Scutellariae and Compound Cordyceps Capsules. Methods: YWG C18 column (4.6 mm × 250 mm, 10 μm); mobile phase acetonitrile water (1:4); detection wavelength: 32 nm, reference wavelength 380 nm; flow rate: 1 ml·min-1; column temperature: Room temperature. RESULTS: The 2,3,5,4’-tetrahydroxystilbene 2 O β D glucoside reference substance was in the range of 1.6 5 - 3.85 μg. The linear relationship between the sample volume and the peak area was good. r = 0 For .9997, the average recovery rate was (98.84 ± 0.23) %. The peak purity factor of the reference product in the compound Cordyceps Capsule was 999.35 2 . Conclusion: The HPLC method was used to determine the content of 2, 3, 5, and 4′-tetrahydroxy glucoside in Shouwu and Compound Cordyceps Capsules. The method was simple and accurate, and could be used to control the preparation of Shouwu and Compound Cordyceps Capsules. the quality of.