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目的:探讨 PBMC 在 HBV 再感染和选择 HBV 变异株中的作用。方法:两名肝移植受者移植前后的血清,PBMC和肝脏中 HBV 的 Pre c/c 和 Pre s/s 基因的 PCR 产物,经克隆后测序,与 ayw 亚型作比较。结果:1.每个标本平均测序了7个克隆,共测序了146个克隆。2.同一个体内,感染肝外部位的 HBV 基因序列不同于感染肝细胞的 HBV 基因序列。3.血清中 HBV 基因序列或发现于肝细胞的 HBV 克隆中,或见于 PBMC 的 HBV 克隆中。4.移植前仅仅存在于 PBMC 中的HBV 变异株,成为移植后感染肝细胞、血及 PBMC 共同的主导株。结论:1.HBV 可在 PBMC 中复制、产生具有感染性的完整的病毒颗粒。2.肝外部位的 HBV 复制,在形成循环的病毒颗粒中具有不可忽视的作用。3.PBMC 中的 HBV 可以成为 HBV 再感染的来源。4.HBV 感染 PBMC 导致选择逃避免疫的 HBV 变异株。
Objective: To investigate the role of PBMC in HBV re-infection and HBV variant selection. Methods: The PCR products of HBV Pre c / c and Pre s / s genes in serum, PBMC and liver of two liver transplant recipients were cloned and sequenced to compare with ayw subtypes. Results: 1. An average of 7 clones were sequenced for each specimen and a total of 146 clones were sequenced. 2. In the same individual, the sequence of the HBV gene that infects the outer part of the liver is different from the sequence of the HBV gene that infects the liver cells. 3. Serum HBV gene sequences or HBV clones found in hepatocytes or in PBMC clones of HBV. 4. Pre-transplant only exists in the PBMC of HBV variants, became infected host liver cells, blood and PBMC common dominating strain. HBV can be replicated in PBMC to produce infectious virus particles. 2. Extrahepatic HBV replication, in the formation of circulating virus particles can not be ignored. 3. HBV in PBMC can become a source of HBV re-infection. Infection of PBMC with HBV resulted in the selection of HBV variants that evaded immunity.