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目的:通过观察4周模拟海拔3000米高住高练低训(HiHiLo)过程中红细胞CD35数量及红细胞C3bRR、ICR的变化,探讨HiHiLo对机体红细胞CD35数量及活性的影响。方法:将16名足球专项运动员随机分为实验组(高住高练低训)和对照组(低住低训)。实验组每晚入住低氧房(O2浓度14.2%,相当于海拔3000米高度)10小时,每周2次低氧房内72%VO2max蹬功率自行车运动30分钟;对照组每周2次正常条件下80%VO2max蹬功率自行车运动30分钟。两组每周同时进行3次由同一教练执导的专项训练。实验期共4周。分别于实验前、入住低氧房10小时、实验2周末、3周末和4周末清晨抽取受试者肘静脉血,采用流式细胞仪检测红细胞CD35几何平均荧光强度,采用红细胞免疫花环试验检测红细胞C3b受体花环率(RBC-C3bRR)和红细胞免疫复合物花环率(RBC-ICR)。结果:实验4周后,实验组和对照组CD35数量较实验前分别下降了4.9%和10.5%(P<0.05),红细胞C3b受体花环率较实验前分别下降了16.7%和24.9%(P<0.01),红细胞IC花环率较实验前分别升高了29.9%(P<0.05)和32.4%。结果表明:(1)HiHiLo对人体红细胞CD35数量的影响不如对红细胞CD35活性的影响明显;(2)HiHiLo影响RBC-C3bRR和RBC-ICR较低住低训更明显,HiHiLo3周后实验组运动员出现临床上的继发性免疫低下现象,4周末有所好转。
OBJECTIVE: To investigate the effect of HiHiLo on the number and activity of CD35 on erythrocytes in HiHiLo rats during 4 weeks of simulated HiHiLo. Methods: Sixteen football special athletes were randomly divided into experimental group (high living high training low training) and control group (low living low training). The experimental group was admitted to hypoxia room every night (O2 concentration 14.2%, equivalent to an altitude of 3000 meters) for 10 hours, twice a week hypoxia room 72% VO2max pedaling power cycling 30 minutes; control group 2 times a week normal conditions Under 80% VO2max pedaling power cycling 30 minutes. The two groups conducted three times a week at the same time directed by the same coach training. The experiment period is 4 weeks. The elbow venous blood samples were collected from the ventricles of the hypoxia for 10 hours, 2 weeks, 3 weeks and 4 weeks before the experiment. The geometric mean fluorescence intensity of CD35 was detected by flow cytometry. The erythrocyte immunocytochemistry C3b receptor rosette rate (RBC-C3bRR) and erythrocyte immune complex rosette rate (RBC-ICR). Results: After 4 weeks of experiment, the number of CD35 in experimental group and control group decreased by 4.9% and 10.5%, respectively (P <0.05), and the rate of C3b receptor rosette in erythrocyte decreased by 16.7% and 24.9% <0.01). The IC rosette rate of erythrocytes increased by 29.9% (P <0.05) and 32.4% respectively compared with those before experiment. The results showed that: (1) The effect of HiHiLo on the number of CD35 of erythrocytes was not as significant as that of CD35 on erythrocytes. (2) The effect of HiHiLo on RBC-C3bRR and RBC- Clinical secondary immunocompromised phenomenon, 4 weeks improved.