论文部分内容阅读
[目的]建立高效液相色谱分离测定0.5%黎芦碱水剂中有效成分含量的分析方法。[方法]采用Eclipse XDB-C_(18)柱和示差折光检测器,以乙腈和0.1%三乙胺水溶液为流动相,测定0.5%黎芦碱水剂中黎芦碱的含量。[结果]黎芦碱在0.2~1 g/L质量浓度范围内线性关系良好,相关系数为0.9996;标准偏差为0.0019;RSD为0.37%;平均回收率99.8%。[结论]该方法操作简便,分离效果好,具有较高的精密度与准确度,可用于黎芦碱含量的测定。
[Objective] To establish an analytical method for the determination of active ingredients in 0.5% [Method] The content of seretide in 0.5% seretide agent was determined by using Eclipse XDB-C 18 column and differential refractive index detector with acetonitrile and 0.1% triethylamine as mobile phase. [Result] The results showed that the linearity of the product was 0.2 ~ 1 g / L, the correlation coefficient was 0.9996, the standard deviation was 0.0019, the RSD was 0.37% and the average recovery was 99.8%. [Conclusion] The method was simple and easy to operate with good separation effect, high precision and accuracy, and could be used for the determination of the content of.